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PHYLOGENETIC GROUPING OF PYRICULARIA ORYZAE ISOLATES ON THE BASIS OF RANDOM AMPLIFIED POLYMORPHIC DNA PROFILES

Hong, Sun-Min (Department of Agricultural Biology) , Bae, Dong-Won (Department of Agricultural Biology) , Kim, Mee Hyang (Department of Agricultural Biology) , Shim, Chang-Ki (Department of Agricultural Biology) , Kang, Kyu-Young (Department of Agricultural Chemistry, Gyeongsang National University) , Kim, Nam-Soo (Department of Agronomy, Kangweon National University) , Kang, Soo-Woong (Gyeongnam Provincial Rural Development Adminstration) , Kim, Hee-Kyu (Department of Agricultural Biology)

Plant molecular biology and biotechnology research center Annual report, 1994, 1994, 337-345

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초록/요약moremore
The size of amplified DNA fragment of rice blast fungi by RAPD raged from 0.5Kb to 4Kb, and the number of these were between 1 and 12. Overall, about 460 polymorphic DNA fragments were generated among each of isolates. The number RAPD products increased with increasing GC content of the primers. Al...
The size of amplified DNA fragment of rice blast fungi by RAPD raged from 0.5Kb to 4Kb, and the number of these were between 1 and 12. Overall, about 460 polymorphic DNA fragments were generated among each of isolates. The number RAPD products increased with increasing GC content of the primers. Although coefficients of pairwise data (similarity matrix) and UPGMA dendrogram were different, they were consistent with tendency of the relatively high similarity for KI races. Our results showed that isolates of KI races and those of KJ races distinguished at 70% genetic similarity in the RAPD assay and isolates within races were very stable (Tables 1 and 2, Figures 1, 2 and 3). Morever, this work also showed that genetic similarity of isolates of KI races were higher degree than those of KJ races, compared with 24 isolates of 8races. We suggested that group of KI race has more homogenous genomic background than that of KJ races, as the isolates of KJ races have been endemic for longer period of time than those of KJ races in Korean paddy field.
목차moremore
Ⅰ. Introduction = 337
Ⅱ. Main Contents = 338
Ⅲ. Summary = 344
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Ⅰ. Introduction = 337
Ⅱ. Main Contents = 338
Ⅲ. Summary = 344