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REGENERATION AND TRANSFORMATION OF CHINESE CABBAGE : BRASSICA CAMPESTRIS L. ssp. PEKINENSIS

Lim, Chae-Oh (Plant Molecular Biology and Biotechnology Research Center) , Choi, Young-Ju (Plant Molecular Biology and Biotechnology Research Center, Department of Food and Nutrition, Pusan Women's University) , Lee, Soo-In (Plant Molecular Biology and Biotechnology Research Center, Department of Biochemistry, Gyeongsang National University) , Koo, Ja-Choon (Plant Molecular Biology and Biotechnology Research Center) , Chun, Hyun-Jin (Plant Molecular Biology and Biotechnology Research Center) , Gal, Sang-Wan (Plant Molecular Biology and Biotechnology Research Center) , Heo, Won-Do (Department of Biochemistry, Gyeongsang National University) , Cho, Moo-Je (Plant Molecular Biology and Biotechnology Research Center, Department of Biochemistry, Gyeongsang National University )

Plant molecular biology and biotechnology research center Annual report, 1994, 1994, 313-319

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초록/요약moremore
This study was conducted to determine the more efficient regeneration and transformation system for Chinese cabbage genetic manipulation. Four-day-old hypocotyl segments of five cultivars grown on MS basal medium supplemented with B5 vitamins, 3% sucrose, 2.0mg/l BAP, 1.0mg/l NAA, in the presence of...
This study was conducted to determine the more efficient regeneration and transformation system for Chinese cabbage genetic manipulation. Four-day-old hypocotyl segments of five cultivars grown on MS basal medium supplemented with B5 vitamins, 3% sucrose, 2.0mg/l BAP, 1.0mg/l NAA, in the presence of 5mg/l AgNO_(3) were not induceded shoots. The comperison of the ability of regeneration between five Chinese cabbage cultivars, shoot induction of Chinese cabbage species seemed to be cultivar-dependent. The best responder was ""Manjum"" variety of four days-old hypocotyl segments. Transformation performed the co-cultivation of hypocotyl segments with A. tumefaciens EHA101 containing the binary vector pGA663 with chloramphenicol acetyltransferase (CAT) as a repoter gene and neomycin phosphotransferase Ⅱ (NPT Ⅱ) as the selectable marker. Transformants were confirmed by the CAT enzyme activities which determined that the CAT gene had been transferred into the plant meterial and was being expressed at leaf and root.
목차moremore
Ⅰ. Introduction = 313
Ⅱ. Main Contents = 314
Ⅲ. Summary = 318
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Ⅰ. Introduction = 313
Ⅱ. Main Contents = 314
Ⅲ. Summary = 318