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TRANSGENIC CITRUS PLANT EXPRESSING δ-ENDOTOXIN GENE FROM B.T.TENEBRIONIS AND ISOLATION OF A NEWSTRAIN OF BACILLUS THURINGIENSIS

Rhim, Seong-Lyul (Hallym University) , Kim, Won-Shik (Hallym University) , Hwang, Li-Chun (Academia Cinica Teipei)

Plant molecular biology and biotechnology research center Annual report, 1994, 1994, 299-305

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초록/요약moremore
A modified crystal δ-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis producing a coleopteran insect-specific toxin was utilized to construct a chimeric gene capable of expressing in plant cells. Using an Agrobacterium tumefaciens binary vector system, the chimeric toxin gene was transferred into citrus leaf disks and recombinant plants were regenerated. Hybridization experiments demonstrated that these plants had the modified toxingene and expressed the insect control toxin protein gene. It is under study whether such expression in the citrus plants can type of Bacillus thuringiensis from soil. From a series of biochemical analysis, we assume that the strain isolated may be included in a new type of the Bacillus thuringiensis subsp. tenebrionis.
A modified crystal δ-endotoxin gene of Bacillus thuringiensis subsp. tenebrionis producing a coleopteran insect-specific toxin was utilized to construct a chimeric gene capable of expressing in plant cells. Using an Agrobacterium tumefaciens binary vector system, the chimeric toxin gene was transferred into citrus leaf disks and recombinant plants were regenerated. Hybridization experiments demonstrated that these plants had the modified toxingene and expressed the insect control toxin protein gene. It is under study whether such expression in the citrus plants can type of Bacillus thuringiensis from soil. From a series of biochemical analysis, we assume that the strain isolated may be included in a new type of the Bacillus thuringiensis subsp. tenebrionis.
목차moremore
Ⅰ. Introduction = 299
Ⅱ. Results and Discussion = 299
Ⅲ. Summary = 304
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Ⅰ. Introduction = 299
Ⅱ. Results and Discussion = 299
Ⅲ. Summary = 304