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MOLECULAR CHARACTERIZATION OF A cDNA ENCODING CYSTEINE PROTEINASE INHIBITOR FROM CHINESE CABBAGE (BRASSICA CAMPESTRIS L. ssp.PEKINENSIS) FLOWER BUDS

Lim, Chae-Oh (Plant Molecular Biology and Biotechnology Research Center) , Lee, Soo-In (Plant Molecular Biology and Biotechnology Research Center, Department of Biochemistry, Gyeongsang National University) , Lee, Sang-Hyeung (Plant Molecular Biology and Biotechnology Research Center) , Kim, Jong-Chel (Plant Molecular Biology and Biotechnology Research Center) , Choi, Young-Ju (Plant Molecular Biology and Biotechnology Research Center, Department of Food and Nutrition, Pusan Women's University) , Bahk, Jeong-Dong (Plant Molecular Biology and Biotechnology Research Center, Department of Biochemistry, Gyeongsang National University) , Lee, Sang-Yeol (Plant Molecular Biology and Biotechnology Research Center, Department of Biochemistry, Gyeongsang National University) , Hwang, In-Hwan (Plant Molecular Biology and Biotechnology Research Center) , Hong, Jong-Chan (Plant Molecular Biology and Biotechnology Research Center, Department of Biochemistry, Gyeongsang National University) , Cho, Moo-Je (Plant Molecular Biology and Biotechnology Research Center, Department of Biochemistry, Gyeongsang National University )

Plant molecular biology and biotechnology research center Annual report, 1994, 1994, 291-297

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초록/요약moremore
A cDNA clone encoding a cysteine proteinase inhibitor was obtained from ESTs of Chinese cabbage flower buds. The cDNA clone spans 784 base pairs, encoded 199-amino acid residues. BCPI contains the conserved Q-V-V-A-R sequence that exists in most members of the cystatin superfamily. Northern blot analysis showed that the amount of mRNA for BCPI was transcribe into a single mRNA species of about 800 nucleotides. BCPI was highly expressed in flower bud. Genomic southern blot analysis showed that BCPI is encoded by a small multigene family consist of at least two members. The protein expressed on E. coli inhibited strongly enzymatic activity of papain, a cysteine proteinase.
A cDNA clone encoding a cysteine proteinase inhibitor was obtained from ESTs of Chinese cabbage flower buds. The cDNA clone spans 784 base pairs, encoded 199-amino acid residues. BCPI contains the conserved Q-V-V-A-R sequence that exists in most members of the cystatin superfamily. Northern blot analysis showed that the amount of mRNA for BCPI was transcribe into a single mRNA species of about 800 nucleotides. BCPI was highly expressed in flower bud. Genomic southern blot analysis showed that BCPI is encoded by a small multigene family consist of at least two members. The protein expressed on E. coli inhibited strongly enzymatic activity of papain, a cysteine proteinase.
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Ⅰ. Introduction = 291
Ⅱ. Main Contents = 292
Ⅲ. Summary = 297
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Ⅰ. Introduction = 291
Ⅱ. Main Contents = 292
Ⅲ. Summary = 297