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RIBOSOME-INACTIVATING PROTEIN GENES FOR USE AS ANTIFUNGAL AGENTS IN TRANSGENIC RICE

Kim, Ju-Kon (Agricultural Biotechnology Institute, Rural Development Administration) , Wu, Ray (Section of Biochemistry, Molecular and Cell Biology, Cornell University) , Hwang, Young-Soo (Agricultural Biotechnology Institute, Rural Development Administration)

Proceedings the 2nd Korean-Germany joint symposium in plant biotechnology, 1994, 1994, 153-161

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초록/요약moremore
Ribosome-inactivating proteins (RIPs) are known to have cytotoxic activity on eucaryotic cells by cleaving a specific adenine residue of 28S rRNA. To test if RIP can be used as antifungal agents in transgenic rice, two chimeric genes containing the maize ribosome-inactivating protein gene, Zmcrip3a,...
Ribosome-inactivating proteins (RIPs) are known to have cytotoxic activity on eucaryotic cells by cleaving a specific adenine residue of 28S rRNA. To test if RIP can be used as antifungal agents in transgenic rice, two chimeric genes containing the maize ribosome-inactivating protein gene, Zmcrip3a, under the control of the rice Actl (pARP7) and the rbcS promoters (pBY605RR) were introduced into Oryza sativa cv Nipponbare by using particle bombardment. After selection of phosphinotricin-resistant calli, regeneration into plants, and genomic DNA gel-blot analysis of the primary transformants, thirteen and seventeen independent fertile transgenic plants for the pARP7 and the pBY605RR constructs, respectively, were obtained. Immunoblot analysis using an anti-maize RIP polyclonal antibody showed that all of the plants analyzed contain the expected size (34 kDa) of the expressed RIP protein and the amount of the expressed protein is approximately 0.5% of total soluble protein in the leaves of the best expressing transgenic plants.
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Ⅰ. Abstract
Ⅱ. Introduction
Ⅲ. Materials and Methods
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Ⅰ. Abstract
Ⅱ. Introduction
Ⅲ. Materials and Methods
Ⅳ. Results and Discussion