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단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석

Proteomic Analysis of Cytokinin Induced Proteins in Arabidopsis,

양영실 (Liang, Ying-Shi, 경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작연구소) , 차준영 (Cha, Joon-Yung, 경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작연구소, 환경생명과학 국가핵심 연구센터) , 네티 엘마와티 (Netty Ermawati, 경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작연구소) , 정민희 (Jung, Min-Hee, 경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작연구소) , 배동원 (Bae, Dong-Won, 경상대학교 대학원 공동실험실습관) , 이창원 (Lee, Chang-Won, 경상대학교 대학원 미생물학과) , 손대영 (Son, Dae-Young, 경상대학교 대학원 응용생명과학부, 식물분자생물학 및 유전자조작연구소, 환경생명과학 국가핵심 연구센터)

Plant molecular biology and biotechnology research center Annual report, 2005, 2005, 389-394

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Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin tr...
Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.