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Production of Tropane Alkaloids by Optimal Culture Conditions in Adventitious Root of Hyoscyamus niger L.

Park, Dong-Jin (Division of Forest Science, Gyeongsang National University) , Min, Ji-Yun (Division of Forest Science, Gyeongsang National University) , Kim, Yong-Duck (Division of Forest Science, Gyeongsang National University) , Kang, Seung-Mi (Division of Forest Science, Gyeongsang National University) , Jung, Ha-Na (Division of Forest Science, Gyeongsang National University) , Kang, Hoon-Serg (Korea Biochemical Co.) , Choi, Myung-Suk (Environmental Biotechnology Research Center, Gyeongsang National University)

Plant molecular biology and biotechnology research center Annual report, 2005, 2005, 369-373

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Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and roots were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best hyoscyamine productivity was resulted in MS medium. Sucrose was increased scopolamine and hyoscyamine production were increased the medium supplemented by sucrose comparing to than those by other carbon sources.
Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and roots were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best hyoscyamine productivity was resulted in MS medium. Sucrose was increased scopolamine and hyoscyamine production were increased the medium supplemented by sucrose comparing to than those by other carbon sources.