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Extracts from Pinus densiflora Siebold et Zuccarini Sawdust Inhibit the Mycelial Growth of Lentinus edodes (Berk.) Sing., Edible Mushroom

Hong, Seong-Cheol (Faculty of Forest Sci. & institute of Agriculture & Life Sci., Gyeongsang Natl. Univ) , Choi, Myung-Suk (Faculty of Forest Sci. & institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.) , Lim, Bu-Kug (Department of Forest Science & Technology, Kyungpook Natl. Univ.) , Lee, Jong-Yoon (Department of Forest Science & Technology, Kyungpook Natl. Univ.) , Danny L. Rinker (Department of Plant Agriculture, University of Guelph) , Yang, JaeK yung (Faculty of Forest Sci. & institute of Agriculture & Life Sci., Gyeong Natl. Univ.)

Plant molecular biology and biotechnology research center Annual report, 2004, 2004, 209-214

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The extracts of Pinus densiflora sawdust by n hexane, ethyl acetate and methanol solvent were investigated to identify their mycelial growth inhibition against Lentinus edodes. The yields of n hexane soluble fraction, ethyl acetate-soluble fraction, and methanol soluble fraction from P. densiflora s...
The extracts of Pinus densiflora sawdust by n hexane, ethyl acetate and methanol solvent were investigated to identify their mycelial growth inhibition against Lentinus edodes. The yields of n hexane soluble fraction, ethyl acetate-soluble fraction, and methanol soluble fraction from P. densiflora sawdust were obtained 1.36%, 2.21% and 4.03% using organic solvent, respectively. The mycelial growth inhibition of L edodes was the greatest for n hexane extract, ranging from 36.5% to 47.6% at concentrations of 125 ppm to 1,000 ppm, with the values for all concentrations significantly different from one another. After direct extraction of P. densiflora sawdust using n hexane, ethyl acetate and methanol, each extract was separated into three fractions by silica gel column chromatography and then the fractions were isolated on the values of R by thin layer chromatography. The mycelial growth inhibition against L. edodes was recognized in the fraction Ⅱ (33.5%) and Ⅲ (37.6%) of n hexane extract, the fraction Ⅱ (21.4%) of ethyl acetate extract and the fraction Ⅱ (26.4%) of methanol extract. The fractions Ⅲ of n-hexane extract showed the highest growth inhibition among the nine fractions of the organic solvent extract.