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Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion

Shin, Dong-jin (Division of Applied Life Science (BK21 Program), and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University) , Koo, Yoon-Duck (Division of Applied Life Science (BK21 Program), and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University) , Lee, Ji-young (Division of Applied Life Science (BK21 Program), and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University) , Lee, Hyo-jung (Division of Applied Life Science (BK21 Program), and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University) , Baek, Dong-won (Division of Applied Life Science (BK21 Program), and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University) , Lee, Suk-han (Department of Genetic Engineering, Sungkyunkwan University) , Cheon, Choong-Ill (Department of Biological Science, Sookmyung Women's University) , Kwak, Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) , Lee, Sang-Yeol (Division of Applied Life Science (BK21 Program), and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University) , Yun, Dae-Jin (Division of Applied Life Science (BK21 Program), and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University)

Plant molecular biology and biotechnology research center Annual report, 2004, 2004, 189-195

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An Arabidopsis cDNA clone that encodes Athb-12, a homeobox-leucine zipper domain protein (HD-Zip), was isolated by functional complementation of the NaC1-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant (cnbΔ, regulatory subunit null). CaN, a Ca^(2+)/calmodulin-dependent protein pho...
An Arabidopsis cDNA clone that encodes Athb-12, a homeobox-leucine zipper domain protein (HD-Zip), was isolated by functional complementation of the NaC1-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant (cnbΔ, regulatory subunit null). CaN, a Ca^(2+)/calmodulin-dependent protein phosphatase, regulates Na+ ion homeostasis in yeast. Expression of Athb-12 increased NaC1 tolerance but not osmotic stress tolerance of these cnbΔ cells. Furthermore, expression of two other HD-Zip from Arabidopsis, Athb-1 and -7, did not suppress NaCl sensitivity of cnbΔ cells. These results suggest that Athb-12 specifically functions in Na+ ion homeostasis in yeast. Consistent with these observations, expression of Athb-12 in yeast turned on transcription of the NaCl stress-inducible PMR2A, which encodes a Na^(+)/Li^(+) translocating P-type ATPase, and decreased Na+ levels in yeast cells. To investigate the biological function of Athb-12 in Arabidopsis, we performed Northern blot analysis. Expression of Athb-12 was dramatically induced by NaCl and ABA treatments, but not by KCl. In vivo targeting experiments using a green fluorescent protein reporter indicated that Athb-12 was localized to the nucleus. These results suggest that Athb-12 is a putative transcription factor that may be involved in NaCl stress reponses in plants.