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Identification of Chinese cabbage genes up-regulated by prolonged cold by using microarray analysis

Yang, Kyung-Ae (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Lim, Chan-Ju (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Hong, Joon-Ki (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Zheng, Lu-Jin (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Hong, Jong-Chan (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Yun, Dae-Jin (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Chung, Woo-Sik (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Lee, Sang-Yeol (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Cho, Moo-Je (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) , Lim, Chae-Oh (Division of Applied Life Scince (BK21), Environmental Biotechnology Research Center and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)

Plant molecular biology and biotechnology research center Annual report, 2005, 2005, 235-242

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Vernalization is induced by prolonged cold and accelerates flowering. To monitor the genome-wide transcriptome change that identifies the genes that are differently up-regulated depending on the duration of cold exposure, we fabricated a Chinese cabbage cDNA microarray and used it to identify genes ...
Vernalization is induced by prolonged cold and accelerates flowering. To monitor the genome-wide transcriptome change that identifies the genes that are differently up-regulated depending on the duration of cold exposure, we fabricated a Chinese cabbage cDNA microarray and used it to identify genes that are up-regulated by short-term (1-week) and long-term (4-week) cold. The overall expression patterns between 1- and 4-week cold treated seedlings were very similar but some genes in the cell growth/division/DNA synthesis functional category were regulated differently. Prolonged cold specifically up-regulated several heat shock proteins (HSPs), and small HSPs were more significant. Among the up-regulated sHSPs, we chose the small HSP 17.6-Ⅱ and confirmed its up-regulation in response to long- but not short-term cold exposure by Northern analysis. And its constitutive overexpression moderately accelerated flowering. ⓒ 2004 Elsevier Ireland Ltd. All rights reserved.