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동충하초의 생리적 특성 및 인공재배에 관한 연구

Studies on the Physiological Characteristics and Artificial Cultivation of Cordyceps,

하남규 (Ha, Nam Gyu, 경상대학교 농과대학원)

원문보기

  • 주제(키워드) 동충하초
  • 발행기관 경상대학교 대학원
  • 지도교수 강진호
  • 발행년도 2006
  • 학위수여년월 2006. 2
  • 학위명 석사
  • 소속대학원 및 학과 대학원 농학과
  • 전공 작물학전공
  • 원문페이지 ii, 35 p.
  • 본문언어 한국어
초록/요약moremore
To develop techniques for the production of Cordyceps stromata on a large scale, morphological and physiological characteristics, and the infection of Bombyx mori with Cordyceps and the growth of stroma cultural characteristics of Cordyceps were investigated. Also, studied about biological activitie...
To develop techniques for the production of Cordyceps stromata on a large scale, morphological and physiological characteristics, and the infection of Bombyx mori with Cordyceps and the growth of stroma cultural characteristics of Cordyceps were investigated. Also, studied about biological activities of fruiting body formed on silkworm. 1. In the morphological aspect, fruiting body of P. tenuipes appeared on Lepidoptera pupa. Stromata was clavate shape and originated simple or occasionally 2 or 5 from host ,and the length was 20~50 ㎜. The spores were similar to an snowflake form. The ascospore of perithecia was cylindrical type and the size was ranged from 3.5~6.0 × 1.5~2.5 ㎛. Cordyceps militaris grown on the pupa of Lepidoptera. Stromata originated simple or occasionally 2 or 3 from host was clavate type and ranged from 9~23 × 6~8 ㎜. Stipe was cylindric form and ranged from 27~30 × 6~7 ㎜ wide. Perithecia subembedded in the stroma was ovoid type and ranged from 465~510 × 260~310 ㎛. Asci were narrowly cylindric. Ascospore was filiform, multiseptate and ranged from 2.2~4.5 × 1.2~1.4 ㎛. 2. In the physiological aspect, mushroom complete medium (MCM) was selected as the most favorable medium for the P. tenuipes and C. militaris. Optimal temperature for the mycelial growth of P. tenuipes and C. militaris was 25℃. 3. In the cultural aspect, mycelial growth of P. tenuipes and C. militaris was favoured by an initial pH 7.0 in the solid medium. In the P. tenuipes, arabinose was the best carbon source and ammonium tartrate was the best nitrogen source. The mycelial growth of C. militaris was most favorable on culture media supplemented with a monosaccharide, glucose as a carbon source. Infection rate of the 5th instar larvae of the silkworm with P. tenuipes was the highest in Yangwonjam, followed by Hachojam, Baegokjam and Chilbojam in that order. Also, as the inoculation times was increased, infection rate tended to be raised. The rate of fruiting body formation of the silkworm pupae infected with P. tenuipes was the highest in Baegokjam, followed by Yangwonjam and Chilbojam in that order. But, actually the fruiting body formation of the 5th instar larvae of the silkworm tested was good in Chilbojam, followed by Yangwonjam and Baegokjam in that order in 3 times spraying inoculation. 4. In the components, mannitol concentration was high in the fruiting bodies of the silkworm infected with C. militaris on a dry weight basis. The fruiting bodies of Yangwonjam and Chilbojam infected with P. tenuipes had high amount of mannitol, but Baegokjam and Hachojam had high concentration of glucose. The most abundant amino acid in the fruiting bodies of the Baegokjam, Chilbojam and Hachojam infected with P. tenuipes was arginine, while Yangwonjam was glycine. C. militaris, had a large amount of glycine, followed by serine.
목차moremore
Ⅰ. 緖言 = 1
Ⅱ. 硏究史 = 3
Ⅲ. 材料 및 方法 = 6
...
Ⅰ. 緖言 = 1
Ⅱ. 硏究史 = 3
Ⅲ. 材料 및 方法 = 6
Ⅳ. 結果 및 考察 = 13
1. 形態的 特性 = 13
2. 生理的 特性 = 15
3. 冬茧夏草의 人工培養 = 20
4. 成分分析 = 23
Ⅴ. 綜合考察 = 29
Ⅳ. 摘要 = 32
引用文獻 = 34